Will steroid medication affect sex hormone levels on Vibrant's Hormones test?

Yes. Oral and inhaled glucocorticoids affect sex hormone function at multiple levels in hypothalamus-pituitary-axis, including the hypothalamus (to decrease the synthesis and release of GnRH), the pituitary gland (to inhibit the synthesis and release of LH and FSH), and the testes/ovary (to modulate steroidogenesis and/or gametogenesis directly).