Of all the Entamoeba spp. detection methods, RT-PCR, which Vibrant uses, has the highest sensitivity for distinguishing E. hystolytica from E. dispar, far outperforming stool microscopy, stool antigen detection, and serology.
The Vibrant publication, Evaluation of the Vibrant DNA Microarray for the High-Throughput Multiplex Detection of Enteric Pathogens in Clinical Samples, reports a sensitivity of 100% (54,1-100% CI), specificity of 100% (99.6-100% CI), positive predictive value of 100% (54.1-100% CI), and negative predictive value of 100% (99.6-100% CI). https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6798489/pdf/13099_2019_Article_329.pdf
The publication provides a detailed overview of Vibrant's pathogen-specific primer design, nucleic acid extraction, multiplex PCR amplification, and array hybridization and target detection methods.
For more information on E. Histolytica prevalence, see Prevalence of Human Intestinal Entamoeba spp. in the Americas: A Systematic Review and Meta-Analysis, 1990–2022. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9699389/pdf/pathogens-11-01365.pdf
It's also worth noting that infectious pathogens are commonly found in the same geographical area and environment where people are exposed to the same water, soil, and air routes of transmission. For more information on this perspective, see: https://vibrantwellness.zendesk.com/hc/en-us/articles/17881664240283-My-patients-seem-to-have-the-same-parasite-detected-frequently-what-could-account-for-that-
