In genetic tests, what does the lowercase c refer to in results (e.g., c.1286A>C or c.665C>T)?

The lowercase “c.” is part of Human Genome Variation Society (HGVS) nomenclature (naming system) and tells you what reference sequence is being used to describe the genetic variant. Specifically, c. = coding DNA sequence.

Example: c.1286A>C

  • c. = coding DNA reference sequence (HGVS nomenclature)
  • 1286 = position in the coding DNA sequence
  • A = reference nucleotide (adenine)
  • >C = changed to cytosine

Read as: “At coding DNA position 1286, an adenine is replaced by a cytosine.”

Example: c.665C>T

  • c. = coding DNA reference
  • 665 = position in the coding sequence
  • C = the reference nucleotide (cytosine)
  • >T = changed to thymine

Read as: “At coding DNA position 665, a cytosine is replaced by a thymine.

Other common HGVS genome prefixes you may see on genetic reports:

  • g. = genomic DNA sequence
  • p. = protein-level change
  • m. = mitochondrial DNA
  • n. = non-coding RNA
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